着丝粒相关蛋白E抗体
规格：1mg/1ml
英文名： CENPE
别名： CENP E; Centromere associated protein E; Centromere autoantigen E (312kD); Centromere autoantigen E; Centromere protein E 312kDa; Centromere protein E; Centromeric protein E; KIF10; Kinesin family mem
分子量： 316kD
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human CENPE
交叉反应：Human,
细胞定位：细胞核
产着丝粒相关蛋白E抗体品介绍：background: Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Nov 2014] Function: Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end-directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. The highly processive microtubule-dependent motor activity of CENPE serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules.着丝粒相关蛋白E抗体 Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss. Required for the efficient recruitment of BUBR1, MAD1 and MAD2 to attached and newly unattached kinetochores. Stimulates mammalian BUBR1 kinase activity. Accumulates just before mitosis at the G2 phase of the cell cycle. Subunit: Monomer. Interacts with CENPF, SEPT7, KIF18A, BUB1B kinase and PRC1. Interacts with NUF2; this interaction determines kinetochore localization. Interacts with SKAP; this interaction greatly favors SKAP binding to microtubules. Subcellular Location: Associates with kinetochores during congression, relocates to the spindle midzone at anaphase, and is 着丝粒相关蛋白E抗体quantitatively discarded at the end of the cell division. Post-translational modifications: The C-terminal inhibitory domain is phosphorylated. Phosphorylation relieves autoinhibition of the kinetochore motor (By similarity Similarity: Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Contains 1 kinesin motor domain. Gene ID: 1062 Database links: Entrez Gene: 1062 Human Omim: 117143 Human SwissProt: Q02224 Human Unigene: 75573 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
着丝粒相关蛋白E抗体产品应用：ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 染色质和核信号 信号转导 细胞分化 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid