髓系/**混合谱系白血病蛋白5抗体
规格：1mg/1ml
英文名： MLL5
别名： HDCMC04P; Histone lysine N methyltransferase MLL5; KMT2E; Lysine N methyltransferase 2E; Myeloid/lymphoid or mixed lineage leukemia 5 (trithorax homolog, Drosophila); Myeloid/lymphoid or mixed lineage
分子量： 205kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human MLL5
交叉反应：Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
细胞定位：细胞核
髓系/**混合谱系白血病蛋白5抗体产品介绍：background: MLL5 is a divergent member of the Drosophila Trithorax related SET and PHD domain containing chromatin regulators that are involved in the regulation of transcriptional "memory" during differentiation. Human MLL5 is located on chromosome 7q22, that is frequently deleted in myeloid leukemias. Inactivation of the Mll5 gene in mice results in a reduction in the average representation of hematopoietic stem cells, and in functional impairment of long term hematopoietic repopulation potential under competitive conditions. Overexpression of the protein also inhibits cell cycle progression.This gene is a member of the myeloid/lymphoid or mixed-lineage leukemia (MLL) family and encodes a protein with an N-terminal PHD zinc finger and a central SET domain. Overexpression of the protein inhibits cell cycle progression. Alternate transcriptional splice variants have been characterized. Function: Histone methyltransferase that specifically mono- and dimethylates 'Lys-4' of histone H3 (H3K4me1 and H3K4me2). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation. Plays an essential role in retinoic-acid-induced granulopoiesis by acting as a髓系/**混合谱系白血病蛋白5抗体 coactivator of RAR-alpha (RARA) in target gene promoters. Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages. Required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells. Overexpression inhibits cell cycle progression, while knockdown induces cell cycle arrest at both the G1 and G2/M phases. Subunit: Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with RARA. Subcellular Location: Nucleus speckle. Note=Absent from the nucleolus. Tissue Specificity: Widely expressed in both ***** and fetal tissues. Highest levels of expression observed in fetal thymus and kidney and in ***** hematopoietic tissues, jejunum and cerebellum. Post-translational modifications: O-glycosylation at Thr-440 in the SET domain by OGT is essential for the histone methyltransferase and the coactivator activity toward RARA in granulopoiesis. The absence of Thr-440 glycosylation in assays done in vitro may explain why some authors did not髓系/**混合谱系白血病蛋白5抗体 detected any histone methyltransferase activity for this protein. Similarity: Belongs to the histone-lysine methyltransferase family. TRX/MLL subfamily. Contains 1 PHD-type zinc finger. Contains 1 SET domain. Database links: UniProtKB/Swiss-Prot: Q8IZD2.1 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
髓系/**混合谱系白血病蛋白5抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 细胞生物 **学 细胞粘附分子
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid