泛素连接酶抗体
规格：1mg/1ml
英文名： PIRH2
别名： Androgen receptor N terminal interacting protein; Androgen receptor N-terminal-interacting protein; ARNIP; CH-rich-interacting match with PLAG1; CHIMP; E3 ubiquitin-protein ligase Pirh2; hARNIP; hPirh
分子量： 30kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human PIRH2
交叉反应：Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
细胞定位：细胞核 细胞浆
泛素连接酶抗体产品介绍：background: Pirh2 has p53-induced ubiquitin-protein ligase activity, promoting p53 degradation. The protein physically interacts with p53 and the resulting degradation of p53 renders Pirh2 an oncogenic protein as the loss of p53 function contributes to malignant tumor development. The gene encoding for the protein maps to chromosome 4q21.1 and transcription of this gene is regulated by p53. Pirh2 expression decreases the level of p53 and a decrease of endogenous Pirh2 expression ups p53 levels. Pirh2 is therefore considered, together with MDM2, to be acting as a negative regulator of p53 function. Function: Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, HDAC1 and CDKN1B. Preferentially acts on tetrameric p53/TP53. Increases AR transcription factor activity 泛素连接酶抗体(By similarity). Contributes to the regulation of CDKN1B and p53/TP53 levels, and thereby contributes to the regulation of the cell cycle progression. Subunit: Monomer and homodimer. Interacts with AR, p53/TP53, MDM2, HDAC1, KAT5, PLAG1, PLAGL2, CDKN1B, COPE, UBE2D2 and GORAB/NTKLBP1 (By similarity). Subcellular Location: Nucleus (By similarity). Nucleus speckle (By similarity). Cytoplasm (By similarity). Tissue Specificity: Detected in testis, liver, kidney and heart. Post-translational modifications: Subject to ubiquitination and proteasomal degradation 泛素连接酶抗体(By similarity). Similarity: Contains 1 CHY-type zinc finger. Contains 1 CTCHY-type zinc finger. Contains 1 RING-type zinc finger. Gene ID: 25898 Database links: Entrez Gene: 771726 Chicken Entrez Gene: 540733 Cow Entrez Gene: 25898 Human Entrez Gene: 68098 Mouse Entrez Gene: 289508 Rat Omim: 607680 Human SwissProt: Q96PM5 Human SwissProt: Q9CR50 Mouse Unigene: 48297 Human Unigene: 159453 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Pirh2通过负反馈调控p53的转录激活和细胞生长抑制功能； Pirh2蛋白是一个新发现的泛素蛋白连接酶，受到p53的诱导调控表达，可促进p53蛋白的泛素化降解。目前多用于肺癌的研究。
泛素连接酶抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 细胞生物 **学 泛素
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid