核抑制蛋白磷酸酶1抗体
规格：1mg/1ml
英文名： NIPP1
别名： Activator of RNA decay; ARD 1; ARD1; Homolog of E.coli RNase E; NIPP 1; NIPP1; Nuclear inhibitor of protein phosphatase 1; Nuclear subunit of PP1; PPP1R8; Protein phosphatase 1 regulatory inhibitor su
分子量： 38kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human NIPP1(25
交叉反应：Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, Guinea Pig, Daniorerio
细胞定位：细胞核 细胞浆
核抑制蛋白磷酸酶1抗体产品介绍：background: Inhibitor subunit of the major nuclear protein phosphatase 1 (PP1). It has RNA binding activity but does not cleave RNA and may target PP1 to RNA associated substrates. May also be involved in pre mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. Function: Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing. Subunit: Interacts with phosphorylated CDC5L, SF3B1 and MELK. Interacts with EED, in a nucleic acid-stimulated manner. Part of a complex consisting of PPP1R8, EED, HDAC2 and PP-1. Part of the spliceosome. Interacts with PPP1CA, PPP1CB and PPP1CC. Subcellular Location: Nucleus. Nucleus speckle. Note=Primarily, but not exclusively, nuclear. Isoform Gamma: Cytoplasm. Note=Found mainly in the cytoplasm. Tissue Specificity: Ubiquitously expressed, with highest levels in heart and核抑制蛋白磷酸酶1抗体 skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform Beta were found in brain, heart and kidney. Isoform Gamma is mainly found in B-cells and T-lymphocytes, and has been found in 293 embryonic kidney cells. Post-translational modifications: May be inactivated by phosphorylation on Ser-199 or Ser-204. Phosphorylated by Lyn in vitro on Tyr-264, and also on Tyr-335 in the presence of RNA. Similarity: Contains 1 核抑制蛋白磷酸酶1抗体FHA domain. Database links: Entrez Gene: 5511 Human Entrez Gene: 100336 Mouse Entrez Gene: 313030 Rat Omim: 602636 Human SwissProt: Q12972 Human SwissProt: Q8R3G1 Mouse Unigene: 533474 Human Unigene: 105230 Mouse Unigene: 16580 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. NIPP1是一种普遍存在于细胞核中的蛋白，它在剪接体的装配中发挥着重要作用，主要体现在细胞的分化，增值及迁移中。经研究，目前NIPP1在乳腺肿瘤、前列腺癌,以及肺癌中有较高频率的表达,其中乳腺肿瘤中的表达频率*高,达到70%,远高于其他肿瘤组织。表明ARD1蛋白的高表达可能是乳腺肿瘤组织的一个标志。
核抑制蛋白磷酸酶1抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 **学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid