多聚合苷酸激酶3磷酸化酶抗体
规格：1mg/1ml
英文名： DEM1/PNK
别名： PNK1; Bifunctional polynucleotide phosphatase/kinase; DEM 1; DEM1; DNA 5' kinase/3' phosphatase; PNK 1; PNK; PNK1; PNKP; Polynucleotide 5' hydroxyl kinase; Polynucleotide kinase 3 prime phosphatase; P
分子量： 57kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human PNK1/PNK
交叉反应：Human, Mouse, Rat, Dog, Cow, Sheep,
细胞定位：细胞核
多聚合苷酸激酶3磷酸化酶抗体产品介绍：background: Mammalian PNK catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. It plays an important function in DNA repair following ionizing radiation or oxidative damage. PNK has been reported to participate in the repair of DNA-double strand breaks via PARP-1-dependent nonhomologous end-joining. Function: Plays a key role in the repair of DNA damage,functioning as part of both the non-homologous end-joining (NHEJ)and base excision repair (BER) pathways. Through its two catalyticactivities, PNK ensures that DNA termini are compatible withextension and ligation by either removing 3'-phosphates from, or byphosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNAbackbone. Subunit: Monomer (By similarity). Subcellular Location: Nucleus. Tissue Specificity: Expressed in many tissues with highestexpression in spleen and testis, and lowest expression in smallintestine (PubMed:10446192). Expressed in higher amount inpancreas, heart and kidney and at lower levels in brain, lung andliver (PubMed:10446193). 多聚合苷酸激酶3磷酸化酶抗体Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. DISEASE: Defects in PNKP are the cause of epilepticencephalopathy, early infantile, type 10 (EIEE10) [MIM:613402]. Adisease characterized by microcephaly, infantile-onset seizures,severe intellectual disability and delayed motor milestones withabsent speech or only achieving a few words. Most patients alsohave多聚合苷酸激酶3磷酸化酶抗体 behavioral problems with hyperactivity. Microcephaly isprogressive and without neuronal migration or structuralabnormalities, consistent with primary microcephaly. Similarity: In the N-terminal section; belongs to the DNA 3'phosphatase family. Contains 1 FHA domain. Gene ID: 11284 Database links: Entrez Gene: 11284 Human Omim: 605610 Human SwissProt: Q96T60 Human Unigene: 78016 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
多聚合苷酸激酶3磷酸化酶抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 细胞生物 **学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid