磷酸化周期素E抗体
规格：1mg/1ml
英文名： phospho-Cyclin E (Thr395)
别名： Cyclin E1 (phospho T395); p-Cyclin E1 (phospho T395); Cyclin E1 (phospho-Thr395); CCNE 1; CCNE;CCNE1;Cyclin E1 antibody Cyclin Es;Cyclin Et;CyclinE; G1/S specific cyclin E; CCNE1_HUMAN.
分子量： 47kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated Synthesised phosphopeptide derived from human
交叉反应：Human,
细胞定位：细胞核
磷酸化周期素E抗体产品介绍：background: Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers. Function: Essential for the control of the cell cycle at the G1/S (start) transition. Subunit: Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex 磷酸化周期素E抗体consisting of UHRF2, CDK2 and CCNE1. Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation. Subcellular Location: Nucleus. Tissue Specificity: Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. Post-translational modifications: Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the cyclin family. Cyclin E 磷酸化周期素E抗体subfamily. Gene ID: 898 Database links: Entrez Gene: 898 Human Entrez Gene: 12447 Mouse Entrez Gene: 25729 Rat Omim: 123837 Human SwissProt: P24864 Human SwissProt: Q61457 Mouse SwissProt: P39949 Rat Unigene: 244723 Human Unigene: 16110 Mouse Unigene: 15455 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 细胞周期素E是调控细胞G-1→S期转变的关键因素。由于在多种肿瘤中的不适当表达,细胞周期素E现在已明确为原癌基因。
磷酸化周期素E抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 细胞生物 神经生物学 细胞凋亡 细胞周期蛋白 新陈代谢
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid