磷酸化真核翻译起始因子4E抗体
规格：1mg/1ml
英文名： phospho-eIF4E (Ser209)
别名： eIF4E (phospho S209); p-eIF4E (phospho S209);eIF4G (phospho-Ser209); eIF4G (phospho-S209); p-eIF4G (Ser209); p-eIF4G (S209); eIF 4 gamma 1; eIF 4G 1; eIF 4G1; EIF4 gamma; EIF4F; EIF4G; EIF4GI; Eukaryo
分子量： 24kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated Synthesised phosphopeptide derived from human
交叉反应：Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Daniorerio
细胞定位：细胞浆
磷酸化真核翻译起始因子4E抗体产品介绍：background: eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk. Function: Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. Subunit: 磷酸化真核翻译起始因子4E抗体eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. Interacts mutually exclusive with EIF4A1 or EIF4A2. Interacts with NGDN and PIWIL2. Component of the CYFIP1-EIF4E-FMR1 complex composed of CYFIP, EIF4E and FMR1. Interacts 磷酸化真核翻译起始因子4E抗体directly with CYFIP1 (By similarity). Interacts with Lassa virus Z protein. Binds to MKNK2 in nucleus. Interacts with LIMD1, WTIP and AJUBA. Subcellular Location: Cytoplasm, P-body. Post-translational modifications: Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex. Similarity: Belongs to the eukaryotic initiation factor 4E family. Gene ID: 1981 Database links: Entrez Gene: 1981 Human Entrez Gene: 208643 Mouse Entrez Gene: 287986 Rat Omim: 600495 Human SwissProt: Q04637 Human SwissProt: Q6NZJ6 Mouse SwissProt: Q4G001 Rat Unigene: 433750 Human Unigene: 260256 Mouse Unigene: 101803 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. EIF4E是蛋白合成的一个主要调控因子，它表达量增加会促进多种肿瘤的生长的增值、分化，因此EIF4E是一种非常磷酸化真核翻译起始因子4E抗体重要的****靶标。
产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：**学 信号转导 细胞凋亡 转录调节因子
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid