磷酸二酯酶5A抗体
规格:1mg/1ml
英文名: PDE5A
别名: 5''-cyclic phosphodiesterase; CGB PDE; CGB-PDE; CGBPDE; cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase; cGMP binding cGMP specific 35 cyclic nucleotide phosphodiesterase; cGMP bi
分子量: 100kDa
储存液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型:Polyclonal
亚型:IgG
纯化方法:affinity purified by Protein A
**原:KLH conjugated synthetic peptide derived from human PDE5A
交叉反应:Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, Guinea Pig,
细胞定位:
磷酸二酯酶5A抗体产品介绍:background: The cGMP specific phosphodiesterase type 5A (PDE5A) family is comprised of 2 genes (PDE5A and PDE5B) each with multiple splice variants generated by RNA splicing and use of alternate initiation sites. The PDE5 is highly expressed in aorta and lungs, intestine, kidney adrenal gland cerebellum and cerebrum. In cerebellum the PDE5 is highly expressed during neonatal development in Purkinge cells layer. The PDE5 is also abundant in vascular smooth muscle regulating cGMP levels and vascular smooth muscle tonicity. In corpus cavernosum inhibition of PDE5 by sildenafil corrects erectile dysfunctions. The nitric oxide donor sodium notropurside (SNP) stimulate PDE5 activity by cGMP dependent kinase phosphorylation. PDE5A1 has 875 amino acids (99.5 kDa). The amino terminal 142 amino acid of 磷酸二酯酶5A抗体the PDE5 gene showed no sequence homology with other PDEs and also contained serine 92 that is phosphorylated by cGMP kinases. Function: Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Tissue Specificity: Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung. Post-translational modifications: Phosphorylation is regulated by binding of cGMP to the 磷酸二酯酶5A抗体two allosteric sites (By similarity). Phosphorylation by PRKG1 leads to its activation. Similarity: Belongs to the cyclic nucleotide phosphodiesterase family. Contains 2 GAF domains. Database links: UniProtKB/Swiss-Prot: O76074.2 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产磷酸二酯酶5A抗体品应用:WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:50-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域:肿瘤 心血管 细胞生物 **学 染色质和核信号 信号转导 新陈代谢
储存条件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源: Rabbit
外观: Lyophilized or Liquid