VP16 tag抗体
规格：1mg/1ml
英文名： VP16 tag
别名： VP16_HHV1F;UL48; Tegument protein VP16; Alpha trans-inducing protein; Alpha-TIF; ICP25; Vmw65
分子量： 54kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from HSV-1 VP16 VP1
交叉反应：Human herpesvirus 1
细胞定位：细胞核
VP16 tag抗体产品介绍：background: HSV evades the immune system through interference with MHC class I antigen presentation on the cell surface, by blocking TAP or the transporter associated with antigen processing induced by the secretion of ICP-47 by HSV. In the host cell, TAP transports digested viral antigen epitope peptides from the cytosol to the endoplasmic reticulum, allowing these epitopes to be combined with MHC class I molecules and presented on the surface of the cell. Viral epitope presentation with MHC class I is a requirement for activation of cytotoxic T-lymphocytes (CTLs), the major effectors of the cell-mediated immune response against virally-infected cells. ICP-47 prevents initiation of a CTL-response against HSV, allowing the virus to survive for a protracted period in the host. Function: Transcriptional activator of immediate-early (IE) gene products (alpha genes). Acts as a key activator of lytic infection by initiating the lytic program through the assembly of the transcriptionalVP16 tag抗体 regulatory VP16-induced complex composed of VP16 and two cellular factors, HCFC1 and POU2F 1. VP16-induced complex represents a regulatory switch: when it is on, it promotes IE-gene expression and thus lytic infection, and when it is off, it limits IE-gene transcription favoring latent infection (By similarity). May play a role in the aggregation of tegument proteins around nucleocapsids during virus morphogenesis. Subunit: Interacts with VP22. Interacts with gH (via C-terminus). Interacts with the virion host shutoff protein (vhs). Interacts with VP11/12. AssociatesVP16 tag抗体 with the VP16-induced complex; binding to host HCFC1 activates VP16 for association with the octamer motif-binding host protein POU2F1, to form a multiprotein-DNA complex responsible for activating transcription of the viral immediate early genes (By similarity). Similarity: Belongs to the herpesviridae tegument protein VP16 protein family. Database links: SwissProt: P04486 Human herpesvirus 1 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产VP16 tag抗体品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：**学 **及病毒
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid