发育和分化增强因子PAG3抗体
规格：1mg/1ml
英文名： PAG3/ASAP2
别名： AMAP 2; AMAP2; ANK repeat and PH domain-containing protein 2; Arf-GAP with SH3 domain; ASAP2; ASAP2_HUMAN; centaurin, beta 3; CENTB3; DDEF 2; DDEF2; Development and differentiation enhancing factor 2;
分子量： 107kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human PAG3
交叉反应：Human, Mouse,
细胞定位：细胞浆
发育和分化增强因子PAG3抗体产品介绍：background: This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript发育和分化增强因子PAG3抗体 variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008] Function: Activates the small GTPases ARF1, ARF5 and ARF6. Regulates the formation of post-Golgi vesicles and modulates constitutive secretion. Modulates phagocytosis mediated by Fc gamma receptor and ARF6. Modulates PXN recruitment to focal contacts and cell migration. Subcellular Location: Cytoplasm. Golgi apparatus > Golgi stack membrane. Cell membrane. Colocalizes with F-actin and ARF6 in phagocytic cups. Tissue Specificity: Detected in heart, brain, placenta, kidney, monocytes and pancreas. Post-translational modifications: Phosphorylated on tyrosine residues by SRC and PTK2B. Similarity:发育和分化增强因子PAG3抗体 Contains 2 ANK repeats. Contains 1 Arf-GAP domain. Contains 1 PH domain. Contains 1 SH3 domain. Database links: Entrez Gene: 8853 Human Entrez Gene: 211914 Mouse Omim: 603817 Human SwissProt: O43150 Human SwissProt: Q7SIG6 Mouse Unigene: 555902 Human Unigene: 358946 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
发育和分化增强因子PAG3抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 激酶和磷酸酶 细胞骨架
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid