胞环蛋白肌动蛋白结合蛋白抗体
规格：1mg/1ml
英文名： Anillin
别名： Actin binding protein scraps homolog Drosophila; Actin binding protein anillin; Anillin actin binding protein; ANLN; DKFZp779A055; Scra; Scraps; Scraps homolog.
分子量： 124kDa
储存液：Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human Anillin
交叉反应：Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,
细胞定位：细胞核 细胞浆
胞环蛋白肌动蛋白结合蛋白抗体产品介绍：background: Anillin, also known as scraps homolog, is an evolutionarily conserved Actin- binding protein required for cytokinesis that was first identified in Drosophila melanogaster. Anillin is a ubiquitously expressed protein with highest expression levels in the central nervous system. It is predominantly found in the nucleus and it localizes to the cleavage furrow during cytokinesis, forming a ring with the help of Rac GTPase. During cytokinesis, Anillin interacts with CD2AP and functions to concentrate Rho A and maintain the localization of active Myosin. In Anillin knockout cells the cleavage furrow fails to complete ingression. Anillin expression levels fluctuate with the cell cycle, peaking in mitosis. Before the cell exits into G1, Anillin associates with E-cadherin and is ubiquitinated by the anaphase-promoting complex/cyclosome (APC/C). APC/C recognizes the D-box domain at the N-terminal region of Anillin. Anillin is commonly overexpressed in tumors and may serve as a potential biomarker.Anillin is required for cytokinesis. It is essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. Function: Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage胞环蛋白肌动蛋白结合蛋白抗体 furrow ingression. Subunit: Interacts with F-actin. Interacts with CD2AP. May interact with RHOA. Interacts with FZR1/CDH1 during mitotic exit. Subcellular Location: Nucleus. Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Note=Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody. Tissue Specificity: Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. Overexpressed in many tumor types including breast, 胞环蛋白肌动蛋白结合蛋白抗体colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors. Post-translational modifications: Phosphorylated during mitosis. Ubiquitinated, and this requires FZR1/CDH1. Similarity: Contains 1 PH domain. Gene ID: 54443 Database links: Entrez Gene: 54443 Human Entrez Gene: 68743 Mouse Entrez Gene: 363031 Rat SwissProt: Q9NQW6 Human SwissProt: Q8K298 Mouse Unigene: 62180 Human Unigene: 282751 Mouse Unigene: 453238 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
胞环蛋白肌动蛋白结合蛋白抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 细胞周期蛋白 结合蛋白 细胞骨架
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid