磷酸化肌球蛋白磷酸酶抗体
规格：1mg/1ml
英文名： Phospho-MYPT1(Thr696)
别名： Myosin Phosphatase (phospho T696); Myosin Phosphatase (phospho Thr696); p-MYPT1(Thr696); Myosin Phosphatase; M130; MBS; MGC133042; Myosin phosphatase target subunit 1; Myosin phosphatase targeting sub
分子量： 113kDa
储存液：Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human Phospho-
交叉反应：Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit,
细胞定位：细胞浆
磷酸化肌球蛋白磷酸酶抗体产品介绍：background: Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in non muscle cells. The guanosine triphosphate (GTP) bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho associated kinase (Rho kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Therefore Rho appears to inhibit myosin phosphatase through the action of Rho kinase. Function: Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity. Subunit: Interacts (when phosphorylated at Ser-445, Ser-472 and Ser-910) with 14-3-3. Interacts with ROCK1 and ROCK2. Interacts with isoform 1 and 磷酸化肌球蛋白磷酸酶抗体isoform 2 of ZIPK/DAPK3. Interacts with RAF1. Interacts with HIF1AN. Subcellular Location: Cytoplasm. Note=Along actomyosin filaments and stress fibers. Tissue Specificity: Expressed in striated muscles, specifically in type 2a fibers (at protein level). Post-translational modifications: Phosphorylated by CIT (Rho-associated kinase). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR. In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1. Phosphorylation at Ser-445, Ser-472 and Ser-910 by NUAK1 promotes interaction with 14-3-3, leading to inhibit interaction with myosin light chain MLC2, preventing dephosphorylation of MLC2. May be phosphorylated at Thr-696 by DMPK; may inhibit the myosin phosphatase activity. Phosphorylated at Ser-473 by CDK1 during mitosis, creating docking sites for the POLO box domains of PLK1. Subsequently磷酸化肌球蛋白磷酸酶抗体 PLK1 binds and phosphorylates PPP1R12A. Similarity: Contains 6 ANK repeats. Gene ID: 4659 Database links: Entrez Gene: 4659 Human Entrez Gene: 17931 Mouse Entrez Gene: 116670 Rat Omim: 602021 Human SwissProt: O14974 Human SwissProt: Q9DBR7 Mouse SwissProt: Q10728 Rat Unigene: 49582 Human Unigene: 422959 Mouse Unigene: 482714 Mouse Unigene: 162937 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
磷酸化肌球蛋白磷酸酶抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 **学 信号转导 细胞凋亡 转录调节因子
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid