丝氨酸/苏氨酸蛋白激酶MLK3抗体
规格：1mg/1ml
英文名： MLK3
别名： MEKK11; MGC17114; Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; Mixed lineage protein kinase 3; Mlk-3; Mlk3; Protein tyrosine kinase PTK1; PTK1; RHOE; SH3 domain containin
分子量： 93kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human MLK3
交叉反应：Human,
细胞定位：
丝氨酸/苏氨酸蛋白激酶MLK3抗体产品介绍：background: Members of the mixed-lineage kinase (MLK) family (including MLK1, MLK2, MLK3, and dual leucine zipper kinase [DLK]) are serine/threonine protein kinases that are expressed in multiple cell types. MLK3 is activated by phosphorylation in response to stress stimuli (e.g., inflammatory responses, UV, chemical stress) that are coupled to the small GTPase, Cdc42/rac. MLK3 is a multifunctional kinase that plays an essential role in several signaling pathways, including mitogen-activated protein kinase (i.e. activation of JNK and p38), IkappaB/NFkappaB, and p70 S6 kinase. Indeed MLK3 signaling occurs through multiple signaling domains in this protein kinase including (from N- to C-terminal) a glycine-rich domain, Src homology 3 (SH3) domain, a kinase domain, a zipper domain, a Cdc42/rac interactive binding (CRIB) domain and a Pro/Ser/Thr-rich domain. Phosphorylation of MLK3 occurs on multiple residues including threonine 277丝氨酸/苏氨酸蛋白激酶MLK3抗体 and serine 281 within the activation loop of the kinase domain. Function: Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1). Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle. Subunit: Homodimer; undergoes dimerization during activation. Subcellular Location: Cytoplasm, cytoskeleton, centrosome. Note=Location is cell cycle dependent. Tissue Specificity: Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and ***** lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain. Post-translational modifications: Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation 丝氨酸/苏氨酸蛋白激酶MLK3抗体of Ser-555 and Ser-556 is induced by CDC42. Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain. Contains 1 SH3 domain. Gene ID: 4296 Database links: Entrez Gene: 4296 Human Entrez Gene: 26403 Mouse Omim: 600050 Human SwissProt: Q16584 Human SwissProt: Q6P2G4 Human SwissProt: Q80XI6 Mouse Unigene: 502872 Human Unigene: 185026 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
丝氨酸/苏氨酸蛋白激酶MLK3抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 细胞生物 **学 神经生物学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid