磷酸化丙酮酸激酶M2抗体
规格：1mg/1ml
英文名： Phospho-PKM2 (Tyr148)
别名： PKM2 (Phospho Tyr148); PKM2 (Phospho Y148); PK 1; PK 2; PK 3; PK Muscle type; PK1; PK2; Pk3; PKL; PKLR; PKM 2; PKM; PKM2; PYKM; Pyruvate kinase 1; Pyruvate kinase 2/3; Pyruvate kinase 3; Pyruvate kina
分子量： 58kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated Synthesised phosphopeptide derived from human
交叉反应：Human, Mouse, Rat, Pig, Cow, Rabbit, Sheep, Guinea Pig,
细胞定位：细胞核 细胞浆
磷酸化丙酮酸激酶M2抗体产品介绍：background: The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. Function: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive 磷酸化丙酮酸激酶M2抗体dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. Subunit: Monomer and homotetramer. Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. Post-translational modifications: ISGylated. Under hypoxia, hydroxylated by EGLN3. Acetylation 磷酸化丙酮酸激酶M2抗体at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy. Similarity: Belongs to the pyruvate kinase family. Gene ID: 5315 Database links: Entrez Gene: 5315 Human Entrez Gene: 18746 Mouse Entrez Gene: 25630 Rat Omim: 179050 Human SwissProt: P14618 Human SwissProt: P52480 Mouse SwissProt: P11980 Rat Unigene: 534770 Human Unigene: 326167 Mouse Unigene: 405069 Mouse Unigene: 1556 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
磷酸化丙酮酸激酶M2抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 **学 信号转导 细胞周期蛋白 激酶和磷酸酶 新陈代谢
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid