E3泛素连接酶RFWD3抗体
规格：1mg/1ml
英文名： RFWD3
别名： BC027246; E3 ubiquitin protein ligase RFWD3; E3 ubiquitin-protein ligase RFWD3; FLJ10520; MGC27888; RFWD3; RFWD3_HUMAN; Ring finger and WD repeat domain 3; RING finger and WD repeat domain containing
分子量： 85kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human RFWD3
交叉反应：Human, Mouse, Dog, Horse,
细胞定位：细胞核
E3泛素连接酶RFWD3抗体产品介绍：Function: E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on E3泛素连接酶RFWD3抗体ubiquitinated p53/TP53. Subcellular Location: Nucleus. Post-translational modifications: Phosphorylated at Ser-46 and Ser-63 upon DNA damage by ATM or ATR. ATM phosphorylation occurs at early times upon DNA damage, while ATR is E3泛素连接酶RFWD3抗体the major kinase at later times. Phosphorylation by ATM and ATR is required to stabilize p53/TP53. Similarity: Contains 1 RING-type zinc finger. Contains 3 WD repeats. Gene ID: 55159 Database links: Entrez Gene: 55159 Human Omim: 614151 Human SwissProt: Q6PCD5 Human Unigene: 567525 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
E3泛素连接酶RFWD3抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 泛素
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid