SHARP2蛋白抗体
规格：1mg/1ml
英文名： SHARP2
别名： Basic helix loop helix domain containing class B 2; BHLHB 2; BHLHB2; bHLHe40; Class B Basic Helix Loop Helix Protein 2; Class E basic helix loop helix protein 40; Clast5; DEC 1; DEC1; Differentially e
分子量： 45kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
SHARP2蛋白抗体**原：KLH conjugated synthetic peptide derived from human SHARP2
交叉反应：Human, Mouse, Rat, Pig, Cow, Horse, Sheep,
细胞定位：细胞浆
产品介绍：background: This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with ARNTL or compete for E-box binding sites in the promoter of PER1 and repress CLOCK/ARNTL's transactivation of PER1. This gene is believed to be involved in the control of circadian rhythm and cell differentiation. [provided by RefSeq, Feb 2014] Function: Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA and NR1H3/LXRA transactivation activity.SHARP2蛋白抗体 May be involved in the regulation of chondrocyte differentiation via the cAMP pathway Subunit: Homodimer. Heterodimer with BHLHE41/DEC2. Interacts with TCF3/E47. Interacts with ubiquitin-conjugating enzyme UBE2I/UBC9. Interacts with HDAC1, SUMO1, RXRA and ARNTL/BMAL1. Subcellular Location: Nuclear Tissue Specificity: Expressed in cartilage, spleen, intestine, lung, and to a lesser extent in heart, brain, liver, muscle and stomach. Post-translational modifications: Ubiquitinated; which may lead to proteasomal degradation. Sumoylation inhibits its ubiquitination and promotes its negative regulation of the CLOCK-ARNTL/BMAL1 heterodimer transcriptional activator activity. Similarity: Contains 1 bHLH (basic helix-loop-helix) domain; Contains 1 Orange domain. Gene ID: 8553 Database links: Entrez Gene: 8553 Human Entrez Gene: 20893 Mouse Entrez Gene:SHARP2蛋白抗体 79431 Rat Entrez Gene: 324413 Zebrafish Omim: 604256 Human SwissProt: Q5EA15 Cow SwissProt: O14503 Human SwissProt: O35185 Mouse SwissProt: O35780 Rat Unigene: 171825 Human Unigene: 2436 Mouse Unigene: 81055 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
SHARP2蛋白抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：转录调节因子 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid