Ras蛋白特异鸟嘌呤核苷酸释放因子4抗体
规格：1mg/1ml
英文名： RASGRP4
别名： RASGRP-4; GRP4_HUMAN; Guanyl nucleotide releasing protein 4; RAS guanyl releasing protein 4; RAS guanyl-releasing protein 4; RASGRP 4.
分子量： 75kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human RASGRP4
交叉反应：Human, Mouse, Dog, Cow, Horse, Sheep,
细胞定位：细胞浆 细胞膜
Ras蛋白特异鸟嘌呤核苷酸释放因子4抗体产品介绍：background: The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009] Function: Functions as a cation- and diacylglycerol (DAG)-regulated nucleotide exchange factor activating Ras through the exchange of bound Ras蛋白特异鸟嘌呤核苷酸释放因子4抗体GDP for GTP. May function in mast cells differentiation. Subcellular Location: Cytoplasm. Cell membrane. Recruited to membranes upon activation by DAG. Tissue Specificity: Expressed by mast cells and their progenitors (at protein level). Specifically expressed in mononuclear leukocytes. Highly expressed in myeloid cells compared to lymphoid cells. Also detected in heart, skeletal muscle, spleen, liver, placenta and lung. Not detected in brain. Isoform 1 is the major isoform in normal individuals. Isoform 2 is more significantly expressed in a patient with asthma. Isoform 3 is more significantly expressed in a patient with asthma and a mastocytosis patient. Similarity: Belongs to the RASGRP family. Contains 1 Ras蛋白特异鸟嘌呤核苷酸释放因子4抗体EF-hand domain. Contains 1 N-terminal Ras-GEF domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 Ras-GEF domain. Gene ID: 115727 Database links: Entrez Gene: 115727 Human Entrez Gene: 233046 Mouse Entrez Gene: 170668 Rat Omim: 607320 Human SwissProt: Q8TDF6 Human SwissProt: Q8BTM9 Mouse SwissProt: Q8R5I4 Rat Unigene: 130434 Human Unigene: 465324 Mouse Unigene: 213071 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Ras蛋白特异鸟嘌呤核苷酸释放因子4抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 信号转导 G蛋白偶联受体 G蛋白信号
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid