SIAH结合蛋白1抗体
规格：1mg/1ml
英文名： PUF60
别名： 60 kDa poly(U)-binding-splicing factor; FBP-interacting repressor; FIR; FLJ31379; FUSE-binding protein-interacting repressor; Poly U binding splicing factor 60KDa; Poly(U)-binding-splicing factor PUF6
分子量： 60kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human PUF60
交叉反应：Human, Mouse, Rat,
细胞定位：细胞核
SIAH结合蛋白1抗体产品介绍：background: This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012] Function: DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced SIAH结合蛋白1抗体apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA. Subcellular Location: Nucleus. Colocalizes partially with TROVE2. Tissue Specificity: Isoform 2 is expressed in colonic epithelium and colorectal epithelium cancer (at protein level). Isoform 6 is expressed in colorectal epithelial cancer but below detection level in colonic epitheliumSIAH结合蛋白1抗体. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Similarity: Belongs to the RRM half pint family. Contains 3 RRM (RNA recognition motif) domains. Gene ID: 22627 Database links: Entrez Gene: 22827 Human Entrez Gene: 426402 Chicken Entrez Gene: 512824 Cow Entrez Gene: 67959 Mouse Entrez Gene: 84401 Rat Entrez Gene: 415211 Zebrafish Omim: 604819 Human SwissProt: Q2HJG2 Cow SwissProt: Q9UHX1 Human SwissProt: Q3UEB3 Mouse SwissProt: Q9WV25 Rat Unigene: 521924 Human Unigene: 29965 Mouse Unigene: 96174 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
SIAH结合蛋白1抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 转录调节因子 结合蛋白 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid