WEE1蛋白抗体
规格：1mg/1ml
英文名： WEE1 protein
别名： DKFZp686I18166; EC 2.7.10.2; FLJ16446; Wee 1; Wee1 homolog; WEE1 homolog S. pombe; Wee1 like protein kinase; Wee1 tyrosine kinase; Wee1+ homolog; Wee1+ S. pombe homolog; WEE1A; Wee1A kinase; WEE1hu; W
分子量： 72kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human WEE1 pro
交叉反应：Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit,
细胞定位：细胞核
WEE1蛋白抗体产品介绍：background: This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. Function: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated.WEE1蛋白抗体 A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. Subcellular Location: Nucleus. Post-translational modifications: Phosphorylated during M and G1 phases. Also autophosphorylated. Phosphorylation at Ser-642 by BRSK1 and BRSK2 in post-mitotic neurons, leads to down-regulate WEE1 activity in polarized neurons. Phosphorylated at Ser-53 and Ser-123 by PLK1 and CDK1, respectively, generating an signal for degradation that can be recognized by the SCF(BTRC) complex, leading to its ubiquitination and degradation at the onset of G2/M phase. Ubiquitinated and degraded at the onset of G2/M phase. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase familyWEE1蛋白抗体. WEE1 subfamily. Contains 1 protein kinase domain. Gene ID: 7465 Database links: Entrez Gene: 7465 Human Entrez Gene: 22390 Mouse Entrez Gene: 308937 Rat Omim: 193525 Human SwissProt: P30291 Human SwissProt: P47810 Mouse SwissProt: Q63802 Rat Unigene: 249441 Human Unigene: 287173 Mouse Unigene: 208255 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
WEE1蛋白抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 信号转导 激酶和磷酸酶 通道蛋白 转运蛋白
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid