NSMCE1蛋白抗体
规格：1mg/1ml
英文名： NSMCE1
别名： HSPC333; HSPC337; Non SMC element 1 homolog (S. cerevisiae); Non SMC element 1 homolog; NSE1_HUMAN; Non structural maintenance of chromosomes element 1 homolog; NSE1; NSMCE 1.
分子量： 31kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human NSMCE1
交叉反应：Human, Mouse, Rat, Cow,
细胞定位：细胞核
NSMCE1蛋白抗体产品介绍：background: Breaks in double stranded DNA often arise during DNA replication or as a result of exposure to DNA-damaging agents. Quick and accurate repair of these breaks is crucial for cell survival and genomic stability. Structural maintenance of chromosomes (SMC) family members form heterodimeric complexes that modulate sister chromatid cohesion and chromosome condensation during mitosis. SMC5 and SMC6 play a crucial role in DNA repair as they form a complex with six conserved nonSMC subunits, including a ubiquitin E3 ligase NSE1 and a SUMO ligase NSE2. Specifically, this complex is crucial for sister chromatid homologous recombination DNA repair and also for prevention of chromosomal rearrangements. The NSE1 protein contains a RING-like motif that promotes DNA repair functions of the SMC5/SMC6 complex and full deletion of NSE1 is lethal to cells. NSE2 stimulates sumoylation of SMC6 and the DNA repair protein TRAX. Depletion of the NSE2 protein by RNA interference leaves the cell vulnerable to DNA damage-induced apoptosis. Function: Component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting theNSMCE1蛋白抗体 SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Has in vitro ubiquitin ligase activity in presence of NDNL2. Is involved in positive regulation of response to DNA damage stimulus. Subunit: Component of the SMC5-SMC6 complex which consists at least of SMC5, SMC6, NSMCE2, NSMCE1,NSMCE1蛋白抗体 NSMCE4A or EID3 and NDNL2. NSMCE1, NSMCE4A or EID3 and NDNL2 probably form a subcomplex that bridges the head domains of the SMC5:SMC6 heterodimer. Subcellular Location: Nucleus. Chromosome, telomere Post-translational modifications: Ubiquitinated. Similarity: Belongs to the NSE1 family. Contains 1 NSE1-type zinc finger. Gene ID: 197370 Database links: Entrez Gene: 197370 Human Entrez Gene: 67711 Mouse Entrez Gene: 361645 Rat SwissProt: Q8WV22 Human SwissProt: Q9D720 Mouse SwissProt: Q499U6 Rat Unigene: 284295 Human Unigene: 4467 Mouse Unigene: 17063 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
NSMCE1蛋白抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid