核RNA结合蛋白P54抗体
规格：1mg/1ml
英文名： NMT55
别名： 52 kDa subunit; 54 kDa nuclear RNA and DNA binding protein; 54 kDa nuclear RNA- and DNA-binding protein; 55 kDa nuclear protein; DNA binding p52/p100 complex 52 kDa subunit; DNA-binding p52/p100 compl
分子量： 54kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human NMT55
交叉反应：Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
细胞定位：细胞核
核RNA结合蛋白P54抗体产品介绍：background: This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009] Function: DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site (By similarity). Involved in pre-mRNA splicing, probably as an heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. 核RNA结合蛋白P54抗体In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. Nono is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. Subcellular Location: Nucleus. Tissue Specificity: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines. DISEASE: Note=A chromosomal aberration involving 核RNA结合蛋白P54抗体NONO may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;X)(p11.2;q13.1) with TFE3. Similarity: Contains 2 RRM (RNA recognition motif) domains. Gene ID: 4841 Database links: Entrez Gene: 4841 Human Entrez Gene: 53610 Mouse Entrez Gene: 100171449 Orangutan Entrez Gene: 317259 Rat Omim: 300084 Human SwissProt: Q15233 Human SwissProt: Q99K48 Mouse SwissProt: Q5RFL9 Orangutan SwissProt: Q5FVM4 Rat Unigene: 533282 Human Unigene: 700344 Human Unigene: 280069 Mouse Unigene: 8381 Rat Important Note: This product as supplied is intended for research use only, not for use in 核RNA结合蛋白P54抗体human, therapeutic or diagnostic applications.
产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 信号转导 结合蛋白 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid