Ninein样蛋白抗体
规格：1mg/1ml
英文名： NINL
别名： 4930519N13Rik; dJ691N24.1; Gm1004,; Gm1634; mKIAA0980; Ninein like; Ninein-like protein; NINL; NINL_HUMAN; Nlp; RGD1306152; RP23-193L22.3; RP4-691N24.1.
分子量： 156kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human NINL
交叉反应：Human, Mouse, Rat,
细胞定位：细胞浆
Ninein样蛋白抗体产品介绍：background: NINL is a 1,382 amino acid protein that localizes to both the cytoplasm and the centrosome and contains four EF-hand domains. Interacting with ?Tubulin, NLP is involved in microtubule organization in interphase cells and, when overexpressed, causes lysosomal dispersion and interferes with mitotic spindle assembly. NLP is subject to post-translational phosphorylation by Plk, an event which disrupts the association of NLP with centrosomes. Function: Involved in the microtubule organization in interphase cells. Overexpression induces the fragmentation of the Golgi, and causes lysosomes to disperse toward the cell periphery; it also interferes with mitotic spindle assembly. May play a role in ovarian carcinogenesis Subunit: nteracts with gamma-tubulin and TUBGCP4. Interacts with anaphase promoting complex/cyclosome (APC/C). Interacts with CDC20 and FZR1. Isoform 2 interacts with LCA5 and USH2A. Subcellular Location: Cytoplasm > cytoskeleton > centrosome. Cytoplasm. In interphase cells, NINL is transported to the centrosomes by the dynein-dynactin motor complex. During centrosome maturation, PLK1 directly phosphorylates NINL resulting in its release into the cytoplasm. Tissue Specificity: Expressed in KYSE150 esophageal carcinoma, Ninein样蛋白抗体HeLa cervical carcinoma and U2OS osteosarcoma cells. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase (at protein level). Expressed in fetal heart, skeletal muscle, liver, lung and cochlea, and in ***** brain, testis, kidney and retina. Post-translational modifications: Phosphorylated by PLK1 which disrupts its centrosome association and interaction with gamma-tubulin. Ubiquitinated by the APC/C complex leading to its degradation. Similarity: Contains 4 EF-hand domains. Gene ID: 22981 Database links: Entrez Gene: 477006 DogNinein样蛋白抗体 Entrez Gene: 100057245 Horse Entrez Gene: 22981 Human Entrez Gene: 78177 Mouse Entrez Gene: 100519522 Pig Entrez Gene: 311529 Rat Omim: 609580 Human SwissProt: Q9Y2I6 Human SwissProt: Q6ZQ12 Mouse Unigene: 631508 Human Unigene: 120911 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Ninein样蛋白抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 细胞周期蛋白 细胞分化
储存条件： Store at -20 癈 for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid