新霉素磷酸转移酶2抗体
规格:1mg/1ml
英文名: NPTII/Neomycin Phosphotransferase 2
别名: Aminoglycoside 3' phosphotransferase; APH(3') II; APH(3')II; aphA; EC 2.7.1.95; Kan; Kanamycin kinase type II; Kanamycin kinase, type VIII; kanamycin resistance gene; Neo; Neomycin kanamycin phosphotr
分子量: 32kDa
储存液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型:Polyclonal
亚型:IgG
纯化方法:affinity purified by Protein A
**原:KLH conjugated synthetic peptide derived from human Neomycin
交叉反应:Escherichia coli,Reacts with bacteria.
细胞定位:
新霉素磷酸转移酶2抗体产品介绍:background: Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin? G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple t新霉素磷酸转移酶2抗体ransfections are performed in parallel with different plasmids. Similarity: Belongs to the aminoglycoside phosphotransferase family. Database links: SwissProt: P00552 Klebsiella pneumoniae Important Note: This product as supplied is intended for research新霉素磷酸转移酶2抗体 use only, not for use in human, therapeutic or diagnostic applications.
新霉素磷酸转移酶2抗体产品应用:WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域:肿瘤 细胞生物 **及病毒 新陈代谢
储存条件: Store at -20 癈 for one year. Avoid repeated freeze/thaw cycles.
来源: Rabbit
外观: Lyophilized or Liquid