E3泛素连接酶NEDD4结合蛋白1抗体
规格：1mg/1ml
英文名： N4BP1
别名： FLJ31821; KIAA0615; MGC176730; N4BP1; N4BP1_HUMAN; NEDD4 binding protein 1; NEDD4-binding protein 1.
分子量： 100kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human N4BP1
交叉反应：Human, Mouse, Rat, Dog, Pig, Horse, Rabbit,
细胞定位：细胞核
E3泛素连接酶NEDD4结合蛋白1抗体产品介绍：background: N4BP1 is an 896 amino acid protein that belongs to the N4BP1 family. N4BP1 primarily localizes to nucleolus, however it also localizes to PML nuclear bodies when desumoylated. Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary, N4BP1 is phosphorylated upon DNA damage probably by ATM or ATR. N4BP1 interacts with Nedd4, a protein with a wide range of activities including modification of ubiquitin-protein ligase, regulation of membrane channels and potentiating hormone-dependent activation of transcription. N4BP1 inhibits the E3 ubiquitin-protein ligase ITCH, by interacting with the second WW domain of ITCH thereby competing with ITCH's substrates and impairing ubiquitination of substrates. N4BP1 has no obvious transmembrane regions, which raises the possibility that N4BP1 represents a soluble adaptor that could sequester ITCH within the cytosol. The N4BP1 gene maps to chromosomeE3泛素连接酶NEDD4结合蛋白1抗体 16q12.1. Function: Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates. Subcellular Location: Nucleus; nucleolus. Nucleus; PML body. Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated. Tissue Specificity: Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary. Post-translational modifications: Monoubiquitinated by NEDD4. PolyubiquitinatedE3泛素连接酶NEDD4结合蛋白1抗体, leading to its degradation by the proteasome. Sumoylated by SUMO1, abrogating polyubiquitination and subsequent degradation. Desumoylated by SENP1, leading to accumulation in PML nuclear bodies. Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the N4BP1 family. Gene ID: 9683 Database links: Entrez Gene: 9683 Human Entrez Gene: 80750 Mouse Entrez Gene: 291921 Rat SwissProt: O75113 Human SwissProt: Q6A037 Mouse Unigene: 511839 Human Unigene: 25117 Mouse Unigene: 428374 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
E3泛素连接酶NEDD4结合蛋白1抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 转录调节因子 表观遗传学 泛素
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid