微管相关丝氨酸/苏氨酸白激酶样抗体
规格：1mg/1ml
英文名： MASTL
别名： 2700091H24Rik; C88295; FLJ14813; GREATWALL; Greatwall protein kinase; GW; GWL; GWL_HUMAN; hGWL; MAST-L; Mastl; MGC117975; Microtubule associated serine/threonine kinase like; Microtubule-associated se
分子量： 97kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human MASTL
交叉反应：Human, Cow, Horse,
细胞定位：细胞浆
微管相关丝氨酸/苏氨酸白激酶样抗体产品介绍：background: This gene encodes a microtubule-associated serine/threonine kinase. Mutations at this locus have been associated with autosomal dominant thrombocytopenia, also known as thrombocytopenia-2. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Feb 2010] Function: Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation. Subcellular Location: Cytoplasm; cytoskeleton; centrosome. Nucleus. Cleavage furrow. During interphase is mainly nuclear, upon nuclear envelope breakdown localizes 微管相关丝氨酸/苏氨酸白激酶样抗体at the cytoplasm and during mitosis at the centrosomes. Upon mitotic exit moves to the cleavage furrow. Post-translational modifications: Phosphorylation at Thr-741 by CDK1 during M phase activates its kinase activity (By similarity). Maximum phosphorylation occurs in prometaphase. DISEASE: Defects in MASTL are the cause of thrombocytopenia type 2 (THC2) [MIM:188000]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. 微管相关丝氨酸/苏氨酸白激酶样抗体Gene ID: 84930 Database links: Entrez Gene: 84930 Human Entrez Gene: 540206 Cow Entrez Gene: 67121 Mouse Omim: 608221 Human SwissProt: E1C2I2 Chicken SwissProt: E1BFR5 Cow SwissProt: E2RJI4 Dog SwissProt: Q96GX5 Human SwissProt: Q8C0P0 Mouse Unigene: 276905 Human Unigene: 253721 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
微管相关丝氨酸/苏氨酸白激酶样抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 信号转导 激酶和磷酸酶
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid