磷酸化丝氨酸/苏氨酸蛋白激酶MARK1抗体
规格：1mg/1ml
英文名： phospho-MARK1 (Thr215)
别名： MARK1 (phospho T215); MARK 1 (phospho T215); p-MARK1 (phospho T215);KIAA1477; MAP/microtubule affinity regulating kinase 1; MAP/microtubule affinity-regulating kinase 1; MARK 1; MARK; Mark1; MARK1_HUM
分子量： 89kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthesised phosphopeptide derived from human
交叉反应：Human, Mouse, Rat, Pig, Cow, Horse,
细胞定位：细胞浆 细胞膜
磷酸化丝氨酸/苏氨酸蛋白激酶MARK1抗体产品介绍：background: MARK1 is thought to play a role in the stability of the microtubule matrix of the cytoskeleton. MARK1 is activated by phosphorylation of Thr215 by LKB1 in complex with STRAD and MO25. Localized to the cytoskeleton, MARK1 contains one kinase-associated (KA1) domain, one protein kinase domain and one UBA domain. Expressed as three isoforms produced by alternative splicing, MARK1 is found with highest levels in brain, skeletal muscle and heart. Function: Serine/threonine-protein kinase involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2, MAP4 and MAPT/TAU. Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). Subcellular Location: Cell membrane; Peripheral membrane protein. Cytoplasm ?cytoskeleton Tissue Specificity: Highly expressed in heart, skeletal muscle, brain, fetal brain and fetal kidney. Post-translational modifications: Phosphorylation at Thr-613磷酸化丝氨酸/苏氨酸蛋白激酶MARK1抗体 by PRKCZ/aPKC in polarized epithelial cells inhibits the kinase activity By similarity. Phosphorylated at Thr-215 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-215 by TAOK1 activates the kinase activity, leading to phosphorylation and detachment of MAPT/TAU from microtubules. Phosphorylation at Ser-219 by GSK3-beta (GSK3B) inhibits the kinase activity. DISEASE: Genetic variations in MARK1 may be associated with susceptibility to autism. MARK1 is overexpressed in the prefrontal 磷酸化丝氨酸/苏氨酸蛋白激酶MARK1抗体cortex of patients with autism and causes changes in the function of cortical dendrites. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 KA1 (kinase-associated) domain. Contains 1 protein kinase domain. Contains 1 UBA domain. Gene ID: 4139 Database links: Entrez Gene: 4139 Human Entrez Gene: 226778 Mouse Entrez Gene: 117016 Rat Omim: 606511 Human SwissProt: Q9P0L2 Human SwissProt: Q8VHJ5 Mouse SwissProt: O08678 Rat Unigene: 497806 Human Unigene: 7445 Mouse Unigene: 21430 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
磷酸化丝氨酸/苏氨酸蛋白激酶MARK1抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 信号转导 激酶和磷酸酶
储存条件： Store at -20 癈 for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid