冠状病毒刺突糖蛋白抗体
规格：1mg/1ml
英文名： SARS spike glycoprotein
别名： E2; E2 glycoprotein; Human coronavirus spike glycoprotein; Peplomer protein; S; S glycoprotein; SPIKE_CVHSA; Severe acute respiratory syndrome spike glycoprotein; Severe acute respiratory syndrome vir
分子量： 138kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human SARS spi
交叉反应：SARS virus
细胞定位：
冠状病毒刺突糖蛋白抗体产品介绍：background: A novel coronavirus has been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive stranded RNA approximately 27 to 31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2. Function: S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. S2 is a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin冠状病毒刺突糖蛋白抗体 intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Subunit: Homotrimer. Binds to human and palm civet ACE2 and human CLEC4M/DC-SIGNR. Interacts with the accessory proteins 3a and 7a. {ECO:0000269|PubMed:14647384, ECO:0000269|PubMed:14670965, ECO:0000269|PubMed:15194747, ECO:0000269|PubMed:15496474, ECO:0000269|PubMed:16166518, ECO:0000269|PubMed:16840309}. Subcellular Location: Virion membrane {ECO:0000269|PubMed:15831954}; Single-pass type I membrane protein {ECO:0000269|PubMed:15831954}. Host endoplasmic reticulum-Golgi intermediate compartment membrane {ECO:0000250}; Single-pass type I membrane protein冠状病毒刺突糖蛋白抗体 {ECO:0000250}. Host cell membrane {ECO:0000269|PubMed:15831954}; Single-pass type I membrane protein {ECO:0000269|PubMed:15831954}. Note=Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly (By similarity). Some S oligomers are transported to the plasma membrane, where they may mediate cell-cell fusion. {ECO:0000250}. Post-translational modifications: The cytoplasmic Cys-rich domain is palmitoylated. Spike glycoprotein is digested by cathepsin CTSL within endosomes. {ECO:0000269|PubMed:17134730}. Similarity: Belongs to the coronaviruses spike protein family. {ECO:0000305}. Database links: http://www.ncbi.nlm.nih.gov/protein/30173397 UniProtKB/Swiss-Prot: P59594.1 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
冠状病毒刺突糖蛋白抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 **及病毒
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid