脱氧尿嘧啶三磷酸核苷水解酶抗体
规格：1mg/1ml
英文名： dUTPase
别名： Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform; dUTP pyrophosphatase; dUTPase; FLJ20622; Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial; Deoxyuridine triphosphatase
分子量： 20kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human dUTPase
交叉反应：Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,
细胞定位：细胞核 细胞浆
脱氧尿嘧啶三磷酸核苷水解酶抗体产品介绍：background: This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. [provided by RefSeq, Jul 2008]. Function: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor脱氧尿嘧啶三磷酸核苷水解酶抗体 of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. Subunit: Homotrimer. Subcellular Location: Isoform 2: Nucleus. Isoform 3: Mitochondrion. Tissue Specificity: Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein level). Post-translational modifications: Nuclear isoform 2 is phosphorylated in vivo on Ser-11, a reaction that can be catalyzed in vitro by CDC2. Phosphorylation in mature T-cells occurs in a cell cycle-dependent manner. Isoform 3 is 脱氧尿嘧啶三磷酸核苷水解酶抗体not phosphorylated. The initiator methionine is cleaved in isoform 2. Similarity: Belongs to the dUTPase family. Gene ID: 1854 Database links: Entrez Gene: 1854 Human Entrez Gene: 110074 Mouse Entrez Gene: 497778 Rat Omim: 601266 Human SwissProt: P33316 Human SwissProt: P70583 Rat Unigene: 527980 Human Unigene: 282499 Mouse Unigene: 6102 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. dUTPase是机体中重要的水解酶，基本作用是把dUTP水解成dUMP和焦磷酸，不但可以提供给DNA的合成原脱氧尿嘧啶三磷酸核苷水解酶抗体料，还可以降低DNA突变的几率，对细胞遗传起到一定的稳定作用。
产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 **学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid