周期素F抗体
规格：1mg/1ml
英文名： Cyclin F
别名： CCNF; F box only protein 1; FBX1; FBXO1; G2 mitotic specific cyclin F; CCNF_HUMAN.
分子量： 86kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human Cyclin F
交叉反应：Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
细胞定位：细胞核 细胞浆
周期素F抗体产品介绍：background: Cyclin F belongs to the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. Such proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. Function: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication. Subunit: Component of the SCF(CCNF) complex consisting of CUL1, RBX1,周期素F抗体 SKP1 and CCNF. Interacts with CCNB1; interaction is required for nuclear localization of CCNB1. Interacts with CCP110; this nteraction leads to CCP110 ubiquitination and degradation via the proteasome pathway. Subcellular Location: Nucleus. Cytoplasm, cytoskeleton, centrosome, centriole. Note=Localization in the centrosome is rare in S phase cells and increases in G2 cells, Localizes on both the mother and daughter centrioles. Localization to centrosomes is not dependent on CP110. Also localizes to the nucleus. Tissue Specificity: Widely expressed. Post-translational modifications: 周期素F抗体Degraded when the spindle assembly checkpoint is activated during the G2-M transition. Degradation is not dependent on the proteasome or ubiquitin and depends on the C-terminal PEST sequence. Phosphorylated just before cells enter into mitosis. Similarity: Belongs to the cyclin family. Cyclin AB subfamily. Contains 1 cyclin N-terminal domain. Contains 1 F-box domain. Gene ID: 899 Database links: Entrez Gene: 899 Human Entrez Gene: 12449 Mouse Omim: 600227 Human SwissProt: P41002 Human SwissProt: P51944 Mouse Unigene: 1973 Human Unigene: 77695 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Cyclin F属于Fbxs蛋白亚型的一种，表达在不同的细胞增殖周期进程中,主要表达在G2-M。
周期素F抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞周期蛋白
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid