DNA修复酶Ku70/DNA修复酶Ku80抗体
规格：1mg/1ml
英文名： Ku70 + Ku80
别名： 70 kDa subunit of Ku antigen; 86 kDa subunit of Ku antigen; ATP dependent DNA helicase 2 subunit 1; ATP dependent DNA helicase 2 subunit 2; ATP dependent DNA helicase II 70 kDa subunit; ATP dependent
分子量： 70 and 83kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human Ku70 + K
交叉反应：Human, Mouse, Rat,
细胞定位：细胞核
DNA修复酶Ku70/DNA修复酶Ku80抗体产品介绍：background: The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008] Function: The Ku autoantigen is a heterodimer of 70kDa (p70) and 80kDa (p80) proteins. The p70 / p80 dimer is important for function of a 460kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. The Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis. Subunit: Heterodimer of a 70 kDa (XRCC6) and a 80 kDa (XRCC5) subunit. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex. The dimer also associates with NAA15, and this DNA修复酶Ku70/DNA修复酶Ku80抗体complex binds to the osteocalcin promoter and activates osteocalcin expression. In addition, XRCC6 interacts with the osteoblast-specific transcription factors MSX2, RUNX2 and DLX5.Interacts with ELF3. Interacts with XRCC6BP1. The XRCC5/6 dimer associates in a DNA-dependent manner with APEX1. Interacts with CLU (By similarity). Binds to CDK9 isoform 2. Identified in a complex with DEAF1 DNA修复酶Ku70/DNA修复酶Ku80抗体and XRCC5. Interacts with DEAF1 (via the SAND domain); the interaction is direct and may be inhibited by DNA-binding. Subcellular Location: Nuclear Similarity: Belongs to the ku70 family. Contains 1 Ku domain. Contains 1 SAP domain. Gene ID: 2547 Database links: Entrez Gene: 2547 Human Entrez Gene: 7520 Human Entrez Gene: 14375 Mouse Entrez Gene: 22596 Mouse Entrez Gene: 25019 Rat Entrez Gene: 363247 Rat Omim: 152690 Human Omim: 194364 Human SwissProt: P12956 Human SwissProt: P13010 Human SwissProt: P23475 Mouse SwissProt: P27641 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
DNA修复酶Ku70/DNA修复酶Ku80抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 细胞生物 转录调节因子 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid