HERC2 E3泛素蛋白连接酶抗体
规格：1mg/1ml
英文名： HERC2
别名： HECT domain and RCC1 like domain containing protein 2; Hect domain and RLD 2; jdf2; p528; SHEP1; HERC2_HUMAN.
分子量： 527kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human HERC2
交叉反应：Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
细胞定位：细胞核 细胞浆
HERC2 E3泛素蛋白连接酶抗体产品介绍：background: HERC2 is a belongs to the HERC gene family that encodes a group of unusually large proteins, which contain multiple structural domains. All members have at least 1 copy of an N-terminal region showing homology to the cell cycle regulator RCC1 and a C-terminal HECT (homologous to E6-AP C terminus) domain found in a number of E3 ubiquitin protein ligases. Genetic variations in this gene are associated with skin/hair/eye pigmentation variability. Multiple pseudogenes of this gene are located on chromosomes 15 and 16. Function: E3 ubiquitin-protein ligase that regulates ubiquitin-dependent retention of repair proteins on damaged chromosomes. Recruited to sites of DNA damage in response to ionizing radiation (IR) and facilitates the assembly of UBE2N and RNF8 promoting DNA damage-induced formation of 'Lys-63'-linked ubiquitin chains. Acts as a mediator of binding specificity between UBE2N and RNF8. Involved in the maintenance of RNF168 HERC2 E3泛素蛋白连接酶抗体levels. E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of XPA which influences the circadian oscillation of DNA excision repair activity. Subunit: Interacts (when phosphorylated at Thr-4827 and sumoylated) with RNF8 (via FHA domain); this interaction increases after ionizing radiation (IR) treatment. Interacts with XPA. Interacts with NEURL4. Via its interaction with NEURL4, may indirectly interact with CCP110 and CEP97. Subcellular Location: Cytoplasm. Cytoplasm, cytoskeleton, centrosome, centriole. Nucleus. HERC2 E3泛素蛋白连接酶抗体Note=Recruited to sites of DNA damage in response to ionizing radiation (IR) via its interaction with RNF8. May loose association with centrosomes during mitosis. Post-translational modifications: Phosphorylation at Thr-4827 is required for interaction with RNF8. Sumoylated with SUMO1 by PIAS4 in response to double-strand breaks (DSBs), promoting the interaction with RNF8. Similarity: Contains 1 cytochrome b5 heme-binding domain. Contains 1 DOC domain. Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. Contains 1 MIB/HERC2 domain. Contains 19 RCC1 repeats. Contains 6 WD repeats. Contains 1 ZZ-type zinc finger. Database links: Entrez Gene: 8924 Human Omim: 605837 Human SwissProt: O95714 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
HERC2 E3泛素蛋白连接酶抗体产品应用：ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid