5’-三磷酸聚核苷酸抗体
规格：1mg/1ml
英文名： HCE
别名： RNGTT; CAP1A; GTase; GTP--RNA guanylyltransferase; hCAP; HCAP1; HcE; HCE1; MCE1; MCE1_HUMAN; mRNA 5''-triphosphatase; mRNA capping enzyme; mRNA guanyltransferase; mRNA guanylyltransferase; polynucleot
分子量： 69kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human HCE
交叉反应：Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, Sheep,
细胞定位：
5’-三磷酸聚核苷酸抗体产品介绍：background: RNGTT (RNA guanylyltransferase and 5'-phosphatase) is a 597 amino acid protein that localizes to the nucleus and exists as four alternatively spliced isoforms. Expressed in kidney, lung, heart, brain, liver, testis, skin and muscle, RNGTT acts as a bifunctional mRNA-capping enzyme that exhibits RNA 5'-triphosphatase activity at its N-terminus and mRNA guanylyltransferase activity at its C-terminus. Via its catalytic activity, RNGTT facilitates the first two steps of mRNA cap formation, naming the removal of a γ-phosphate from the end of nascent mRNA and the subsequent transfer of the phospho residue to the 5’-diphosphate terminus. The gene encoding RNGTT maps to human chromosome 6, which contains 170 million base pairs and comprises nearly 6% of the human genome. Function: Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. 5’-三磷酸聚核苷酸抗体Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus. Subunit: Interacts with SUPT5H and RNMT. Interacts with HIV-1 Tat. Subcellular Location: Nucleus. Tissue Specificity: Isoform 1 and isoform 4 (at a lesser extent) are expressed in cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin and muscle. Similarity: In the N-terminal section; belongs to5’-三磷酸聚核苷酸抗体 the non-receptor class of the protein-tyrosine phosphatase family. In the C-terminal section; belongs to the eukaryotic GTase family. Gene ID: 8732 Database links: Entrez Gene: 8732 Human Entrez Gene: 24018 Mouse GenBank: AF025654 Human Omim: 603512 Human SwissProt: O60942 Human SwissProt: O55236 Mouse Unigene: 567378 Human Unigene: 240024 Mouse Unigene: 464346 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
5’-三磷酸聚核苷酸抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 **学 染色质和核信号 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid