Pan ras抗体
规格：1mg/1ml
英文名： Ras
别名： Pan-ras; Pan ras; K-Ras; P21 protein; p21ras; H-Ras-1; c-H-ras; N-Ras; c bas/has; c H ras; c has/bas p21 protein; C K RAS; c K ras2 protein; c Ki ras; c Kirsten ras protein; c ras Ki 2 protein; C-BAS/
分子量： 21kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human ras
交叉反应：Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
细胞定位：
Pan ras抗体产品介绍：background: RAS superfamily comprises around 50 related genes encoding GTP-binding domain (G-proteins) involved in signal transduction. The main genes are HRAS, NRAS and KRAS. Ras proteins are membrane-bound GTPases. The inactive form is GDP-bound. They are activated by ligand-binding receptor tyrosine kinases such as EGFR, PDGFR, colony-stimulating factor and fibroblast growth factor. These kinases transiently convert RAS-GDP to RAS-GTP, the active form of RAS. Single amino acid substitutions can activate RAS making it highly oncogenic. Such mutations generally reduce the GTPase activity of RAS, prolonging it in its active GTP-bound form. The consequence of this is sustained activation of the RAF1-MAPK signalling pathway. RAS mutations are found in 10-15% of tumours. A high incidence of RAS mutations is found in pancreatic cancers. Function: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Subcellular Location: Cell membrane. Golgi apparatus membrane. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus. Post-translational modifications: Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi. S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation. DISEASE: Defects in HRAS are the cause of faciocutaneoskeletal syndrome (FCSS) [MIM:218040]. A rare condition characterized by prenatally increased growth, postnatal growth deficiency, mental retardation, distinctive facial appearance, cardiovascular abnormalities (typically pulmonic stenosis, hypertrophic cardiomyopathy and/or atrial tachycardia), tumor predisposition, skin and musculoskeletal abnormalities. Defects in HRAS are the cause of congenital myopathy with excess of musclePan ras抗体 spindles (CMEMS) [MIM:218040]. CMEMS is a variant of Costello syndrome. Defects in HRAS may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms. Note=Mutations which change positions 12, 13 or 61 activate the potential of HRAS to transform cultured cells and are implicated in a variety of human tumors. Defects in HRAS are a cause of susceptibility to bladder cancer (BLC) [MIM:109800]. A malignancy originating in tissues of the urinary bladder. It often presents with multiple tumors appearing at different times and at different sites in the bladder. Most bladder cancers are transitional cell carcinomas. They begin in cells that normally make up the inner lining of the bladder. Other types of bladder cancer include Pan ras抗体squamous cell carcinoma (cancer that begins in thin, flat cells) and adenocarcinoma (cancer that begins in cells that make and release mucus and other fluids). Bladder cancer is a complex disorder with both genetic and environmental influences. Note=Defects in HRAS are the cause of oral squamous cell carcinoma (OSCC). Similarity: Belongs to the small GTPase superfamily. Ras family. Gene ID: 3265 Database links: Entrez Gene: 3265 Human Entrez Gene: 3845 Human Entrez Gene: 4893 Human Entrez Gene: 15461 Mouse Entrez Gene: 16653 Mouse Entrez Gene: 18176 Mouse Entrez Gene: 24525 Rat Entrez Gene: 24605 Rat Entrez Gene: 293621 Rat Omim: 164790 Human Omim: 190020 Human Omim: 190070 Human SwissProt: P01111 Human SwissProt: P01112 Human SwissProt: P01116 Human SwissProt: P08556 Mouse SwissProt: P32883 Mouse SwissProt: Q61411 Mouse SwissProt: P08644 Rat SwissProt: P20171 Rat SwissProt: Q04970 Rat Unigene: 37003 Human Unigene: 486502 Human Unigene: 505033 Human Unigene: 334313 Mouse Unigene: 102180 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. GDP/GTP结合蛋白（GDP/GTP Bijding Protein）
产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other Pan ras抗体applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid