调节蛋白GAL4抗体
规格：1mg/1ml
英文名： GAL4
别名： Gal4p; GAL81; Homo sapiens galectin4 mRNA complete cds; Regulatory protein GAL4; GAL4_YEAST.
分子量： 99kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from Saccharomyces
交叉反应：Saccharomyces cerevisiae
细胞定位：细胞核
调节蛋白GAL4抗体产品介绍：background: The GAL4 protein of Saccharomyces cerevisiae is one of the most thoroughly characterized transcriptional activators. As the N-terminal 147 amino acid residues of GAL4 are sufficient to mediate specific and strong binding to DNA, but are incapable of efficient transcriptional activation, this protein fragment has frequently been used to confer specific DNA binding in experiments examining transcriptional activation functions of heterologous proteins. This approach is facilitated by the finding that higher eukaryotes lack endogenous proteins that enhance transcription from the consensus GAL4-binding site. Fusions between GAL4 (amino acids 1-147) and activating domains from a variety of transcriptional regulatory proteins can activate transcription in yeast, plant, insects and mammalian cells. Fields and coworkers have taken advantage of these findings by the development of a unique “two-hybrid” system using GAL4 fusions in yeast to identify specific protein-protein interactions. Function: This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7,调节蛋白GAL4抗体 GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes. Subunit structure: Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17. Domain: The 9aaTAD motif (residues 862 to 870) is a transactivation 调节蛋白GAL4抗体domain present in a large number of yeast and animal transcription factors. Post-translational modification: Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4. Subunit: Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17. Subcellular Location: Nuclear Post-translational modifications: Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4. Similarity: Contains 1 Zn(2)-C6 fungal-type DNA-binding domain. Database links: Entrez Gene: 855828 Saccharomyces cerevisiae SwissProt: P04386 Saccharomyces cerevisiae Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
调节蛋白GAL4抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 细胞生物 信号转导 转录调节因子 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid