生长因子受体结合蛋白14抗体
规格：1mg/1ml
英文名： GRB 14
别名： GRB14 adapter protein; GRB14; GRB14_HUMAN; Growth factor receptor bound protein 14.
分子量： 61kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human GRB 14
交叉反应：Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, Sheep,
细胞定位：细胞膜
生长因子受体结合蛋白14抗体产品介绍：background: Many growth factors function by binding receptors with intrinsic tyrosine kinase activity. Signaling by such receptors involves a series of intermediates characterized by SH2 domains that bind tyrosine phosphorylated receptors by a direct interaction between the SH2 domain and specific phospho-tyrosine-containing receptor sequences. GRB7, a SH2 domain protein, has a single SH2 domain at its C-terminal, a central region with similarity to Ras GAP and a proline-rich N-terminus. A related SH2 domain-containing protein, GRB10, exhibits a high degree of homology with GRB7. GRB10 undergoes serine but not tyrosine phosphorylation in response to EGF treatment, but appears to bind to the EGF receptor poorly. An additional member of the GRB7 family, designated GRB14, contains a Pleckstrin homology domain in its central region and a carboxy-terminal SH2 domain. GRB14 mRNA is expressed at high levels in a broad range of tissues including liver, kidney, pancreas, testis, ovary, heart and skeletal muscle. Expression of the GRB14 protein in breast carcinomas is strongly correlated with estrogen receptor positivity. Function: The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. This protein likely has an inhibitory effect on receptor tyrosine kinase signaling and, in particular, on insulin receptor signaling. This gene生长因子受体结合蛋白14抗体 may play a role in signaling pathways that regulate growth and metabolism. Transcript variants have been reported for this gene, but their full-length natures have not been determined to date. Subunit: Interacts with the cytoplasmic domain of the autophosphorylated insulin receptor (INSR), through the SH2 domain (By similarity). Interacts with GRB14 (via BPS domain); this interaction protects the tyrosines in the activation loop on INSR from dephosphorylation. Binds to the ankyrin repeat region of TNKS2 via its N-terminus. Interacts with activated NRAS. Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated). Subcellular Location: Cytoplasm, Golgi apparatus membrane, Peripheral membrane 生长因子受体结合蛋白14抗体protein, Endosome membrane. Tissue Specificity: Expressed at high levels in the liver, kidney, pancreas, testis, ovary, heart and skeletal muscle. Post-translational modifications: Phosphorylated on serine residues. Phosphorylated on tyrosine residues by TEK/TIE2. Similarity: Belongs to the GRB7/10/14 family. Contains 1 PH domain. Contains 1 Ras-associating domain. Contains 1 SH2 domain. Gene ID: 2888 Database links: Entrez Gene: 2888 Human Entrez Gene: 50915 Mouse Entrez Gene: 58844 Rat Omim: 601524 Human SwissProt: Q14449 Human SwissProt: Q9JLM9 Mouse SwissProt: O88900 Rat Unigene: 411881 Human Unigene: 214554 Mouse Unigene: 30028 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
生长因子受体结合蛋白14抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 细胞生物 信号转导 激酶和磷酸酶 细胞膜受体
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid