弗林蛋白酶抗体
规格：1mg/1ml
英文名： Furin
别名： Dibasic processing enzyme; Dibasic-processing enzyme; FES upstream region; FUR; FURIN; FURIN_HUMAN; PACE; Paired basic amino acid residue cleaving enzyme; Paired basic amino acid residue-cleaving enzy
分子量： 74kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human Furin
交叉反应：Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,
细胞定位：细胞浆 细胞膜 细胞外基质
弗林蛋白酶抗体产品介绍：background: Furin is a calcium-dependent serine endoprotease that belongs to the subtilisin-like proprotein convertase family. The members of this family process latent precursor proteins into their biologically active products. Furin cleaves at paired basic amino acid processing sites within proparathyroid hormone, transforming growth factor β 1 precursor, proalbumin, pro-β-secretase, membrane type-1 matrix metalloproteinase, β subunit of pro-nerve growth factor and von Willebrand factor. Furin can directly cleave proMMP-2 within the ttrans-Golgi network leading to an inactive form of matrix metalloproteinase-2 (MMP-2). Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. The inhibitory mechanism is based on the presence of an inactivating prosegment at the NH2 terminal of the Furin. After initial autocatalytic cleavage, the prosegment remains tightly associated until it reaches the trans-Golgi network where the dissociation of the prosegment and activation of furin occurs. Function: Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Subunit: Interacts with FLNA (By similarity). Binds to PACS1 which mediates TGN localization and connection to clathrin adapters. Subcellular Location: Golgi apparatus > 弗林蛋白酶抗体trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin. Tissue Specificity: Seems to be expressed ubiquitously. Post-translational modifications: The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation. Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-,弗林蛋白酶抗体 mono- and non-phosphorylated forms. Similarity: Belongs to the peptidase S8 family. Furin subfamily. Contains 1 homo B/P domain. Gene ID: 5045 Database links: Entrez Gene: 5045 Human Entrez Gene: 18550 Mouse Entrez Gene: 54281 Rat Omim: 136950 Human SwissProt: P09958 Human SwissProt: P23188 Mouse SwissProt: P23377 Rat Unigene: 513153 Human Unigene: 5241 Mouse Unigene: 3220 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 在真核生物细胞中,许多具有生物活性的多肽和蛋白是在其分泌过程中由前体蛋白经内切蛋白酶切割后激活形成的.弗林蛋白酶(Furin)就是这个内切蛋白酶家族重要成员之一,它可以识别剪切多种蛋白质,如生长医子、血清蛋白、基质金属蛋白酶、受体、病毒囊膜蛋白和**外**等.近年来Furin得到了迅速而广泛的研究,本文简介了它的表达与加工运输、生物学功能、与病毒侵染的关系,以及它的抑制剂.
弗林蛋白酶抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 信号转导 新陈代谢 泛素
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid