E3泛素蛋白连接酶EDD抗体
规格：1mg/1ml
英文名： UBR5
别名： 4432411E13Rik; AW549941; C77315; D030042K14; DD5; E3 identified by differential display; E3 ubiquitin protein ligase, HECT domain containing, 1; E3 ubiquitin-protein ligase; E3 ubiquitin-protein ligas
分子量： 309kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human EDD
交叉反应：Human, Mouse, Rat, Chicken, Dog, Pig, Horse, Sheep,
细胞定位：细胞核
E3泛素蛋白连接酶EDD抗体产品介绍：background: EDD is a progestin-regulated gene that was isolated from T-47D human breast cancer cells. Based on sequence homology, EDD appears to be a human homolog of the Drosophila hyperplastic discs (hyd) gene, a tumor suppressor gene that is required for control of imaginal disc growth. EDD contains a HECT domain in the carboxy terminus. HECT domain-containing proteins function as ubiquitin-protein ligases, or E3 enzymes. EDD has been shown to bind to ubiquitin, and like other HECT family proteins, may function as an E3 ubiquitin-protein ligase. Function: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradationE3泛素蛋白连接酶EDD抗体 (By similarity). May be involved in maturation and/or transcriptional regulation of mRNA. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development. Subunit: Binds TOPBP1. Associates with CDK9 and TFIIS/TCEA1 and forms a transcription regulatory complex made of CDK9, RNAP II, UBR5 and TFIIS/TCEA1 that can stimulate target gene transcription (e.g. gamma fibrinogen/FGG) byE3泛素蛋白连接酶EDD抗体 recruiting their promoters. Associates with the E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and VPRBP proteins (EDVP complex). Interacts directly with DYRK2. Subcellular Location: Nucleus. Tissue Specificity: Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. Contains 1 PABC domain. Contains 1 UBR-type zinc finger. Gene ID: 51366 Database links: Entrez Gene: 515582 Cow Entrez Gene: 51366 Human Entrez Gene: 70790 Mouse Entrez Gene: 100157625 Pig Entrez Gene: 117060 Rat Omim: 608413 Human SwissProt: O95071 Human SwissProt: Q80TP3 Mouse SwissProt: Q62671 Rat Unigene: 492445 Human Unigene: 476840 Mouse Unigene: 54812 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
E3泛素蛋白连接酶EDD抗体产品应用：ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 表观遗传学 泛素
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid