DNA聚合酶ι/DNA pol ι抗体
规格：1mg/1ml
英文名： DNA Polymerase iota
别名： DNA polymerase iota; Eta 2; Eta2; POLI; POLI_HUMAN; Polymerase (DNA directed) iota; RAD 30B; RAD30 homolog B; RAD30B; RAD3OB.
分子量： 83kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human DNA Poly
交叉反应：Human, Mouse, Rat, Dog,
细胞定位：细胞核
DNA聚合酶ι/DNA pol ι抗体产品介绍：background: DNA polymerase activity is essential for replication, repair, recombination and mutagenesis. DNA polymerases can often bypass DNA lesions that block DNA replication, thereby allowing the replication of damaged DNA. One such DNA polymerase is the distributive enzyme DNA Pol i, which is encoded by the POLI gene. POLI is located on human chromosome 18q21.2, a region often implicated in the etiology of many human cancers. At thymine templates, DNA Pol i is highly error-prone when replicating undamaged DNA in that it favors the misincorporation of guanine over the correct nucleotide, adenosine. DNA Pol i also promotes the replication of damaged DNA by misincorporating deoxynucleotides opposite DNA lesions. DNA Pol i acts sequentially with DNA Pol Ω, which is essential forDNA聚合酶ι/DNA pol ι抗体 damage-induced mutagenesis, to complete the DNA lesion bypass. Therefore, replication involving DNA Pol i is likely to be highly mutagenic. Function: Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially DNA聚合酶ι/DNA pol ι抗体insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity. Subunit: Interacts with REV1 (By similarity). Interacts with POLH. Subcellular Location: Nucleus. Accumulates at replication forks after DNA damage. Tissue Specificity: Ubiquitous. Highly expressed in testis. Similarity: Belongs to the DNA polymerase type-Y family. Contains 1 umuC domain. Database links: UniProtKB/Swiss-Prot: Q9UNA4.3 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
DNA聚合酶ι/DNA pol ι抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 信号转导 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid