酪氨酸激酶C-SRC抗体
规格：1mg/1ml
英文名： CSK
别名： C SRC; C SRC kinase; C src Tyrosine Kinase; C-SRC kinase; c-src tyrosine kinase; Csk A; CSK; CSK_HUMAN; CYTOPLASMIC TYROSINE KINASE; EC 2.7.10.2; MGC112926; MGC117393; MGC154049; P60 Src; Protein tyro
分子量： 51kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human CSK
交叉反应：Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep, Monkey,
细胞定位：细胞浆 细胞膜
酪氨酸激酶C-SRC抗体产品介绍：background: All members of the Src gene family of tyrosine kinases are characterized by a carboxy terminal domain tyrosine which is highly phosphorylated in the inactive form of the enzyme and phosphorylated to a much lesser extent when the enzyme is active. In the case of Src p60, Y527 is this tyrosine; however, a mutant form of c-Src in which Y527 is replaced by phenylalanine is transforming and displays 5- to 10-fold elevated kinase activity compared to its normal counterpart. Csk has been identified as a Src-related tyrosine kinase having both SH2 and SH3 domains and a catalytic domain but lacking sequences amino terminal to the SH3 domain as well as carboxy terminal regulatory sequences. Csk phosphorylates Src on Y527 and also downregulates Lyn, Fyn and Lck by tyrosine phosphorylation of carboxy 酪氨酸激酶C-SRC抗体terminal regulatory sites. Function: Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases. Subunit: Homodimer (via SH3-domain). Interacts with PTPN8 (By similarity). Interacts with phosphorylated SIT1, PAG1, LIME1 and TGFB1I1; these interactions serve to recruit CSK to the membrane where it can phosphorylate and inhibit Src-family kinases. Interacts with SRCIN1. Interacts with RHOH. Interacts (via SH2 domain) with SCIMP. Subcellular Location: Cytoplasm. Cell membrane. Mainly cytoplasmic, also酪氨酸激酶C-SRC抗体 present in lipid rafts. Tissue Specificity: Expressed in lung and macrophages. Post-translational modifications: Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain. Gene ID: 1445 Database links: Entrez Gene: 509246 Cow Entrez Gene: 487649 Dog Entrez Gene: 1445 Human Entrez Gene: 12988 Mouse Entrez Gene: 315707 Rat Omim: 124095 Human SwissProt: Q0VBZ0 Cow SwissProt: P41240 Human SwissProt: P41241 Mouse SwissProt: P32577 Rat Unigene: 77793 Human Unigene: 21974 Mouse Unigene: 470150 Mouse Unigene: 2759 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
酪氨酸激酶C-SRC抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 信号转导 激酶和磷酸酶
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid