E3泛素蛋白连接酶MIB1抗体
规格：1mg/1ml
英文名： Mib1
别名： Mib1/Mindbomb; DAPK-interacting protein 1; Dip 1; DIP-1; Dip1; E3 ubiquitin protein ligase MIB 1; E3 ubiquitin protein ligase MIB1; E3 ubiquitin-protein ligase mib1; mib1; MIB1_HUMAN; Mind bomb homolo
分子量： 110kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human Mib1/Min
交叉反应：Human, Mouse, Rat, Chicken, Dog, Cow, Rabbit,
细胞定位：细胞浆 细胞膜
产品介绍：background: MIB1 is a 1006 amino acid E3 ubiquitin ligase that activates the Notch ligand, Delta. MIB1 ubiquinates Delta by binding to its intracellular domain, leading to the endocytosis and eventual degradation of the Delta receptor, which, paradoxically, results in the up-regulation of receptor activity and enhances Notch signaling. MIB1 also interacts with DAPK, a protein that plays an important role in the regulation of apoptosis. Ubiquination of DAPK leads to inhibition of caspase-dependent apoptosis, therefore it is likely that overexpression of MIB1 can lead to tumor growth. Although it seems to be widely expressed at low levels, MIB1 is expressed at highest concentrations in the CNS and ovary. Both DAPK E3泛素蛋白连接酶MIB1抗体and MIB1 are overexpressed in epileptic brain tissue, suggesting that they probably cooperate as regulators of neuronal death in epilepsy. Function: E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis. Subcellular Location: Cytoplasm. Cell membrane. Localizes to the plasma membrane (By similarity). According to PubMed:15048887, E3泛素蛋白连接酶MIB1抗体it is mitochondrial, however such localization remains unclear. Tissue Specificity: Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined. Post-translational modifications: Ubiquitinated. Possibly via autoubiquitination. Similarity: Contains 9 ANK repeats. Contains 2 MIB/HERC2 domains. Contains 3 RING-type zinc fingers. Contains 1 ZZ-type zinc finger. Database links: Entrez Gene: 57534 Human Entrez Gene: 225164 Mouse Omim: 608677 Human SwissProt: Q86YT6 Human SwissProt: Q80SY4 Mouse Unigene: 140903 Human Unigene: 658808 Human Unigene: 21500 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.E3泛素蛋白连接酶MIB1抗体
产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
E3泛素蛋白连接酶MIB1抗体研究领域：肿瘤 细胞生物 神经生物学 信号转导 干细胞 跨膜蛋白
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid