线粒体DNA拓普西异构酶Ⅰ抗体
规格：1mg/1ml
英文名： TOP1MT
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human TOP1mt
交叉反应：Human, Mouse, Rat,
细胞定位：
线粒体DNA拓普西异构酶Ⅰ抗体产品介绍：background: This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]. Function: Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex.线粒体DNA拓普西异构酶Ⅰ抗体 Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Subcellular线粒体DNA拓普西异构酶Ⅰ抗体 Location: Mitochondrion. Tissue Specificity: Ubiquitous; highest in skeletal muscle, heart, brain and fetal liver. Similarity: Belongs to the type IB topoisomerase family. Gene ID: 116447 Database links: Entrez Gene: 116447 Human Omim: 606387 Human SwissProt: Q969P6 Human Unigene: 528574 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
线粒体DNA拓普西异构酶Ⅰ抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 染色质和核信号 转录调节因子 激酶和磷酸酶 线粒体
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid
别名： Topoisomerase (DNA) I, mitochondrial; mitochondrial DNA topoisomerase I; DNA topoisomerase I, mitochondrial; TOP1mt; 2900052H09Rik; TOP1MT; TOP1M_HUMAN; DNA topoisomerase I, mitochondrial; mitochondri
分子量： 66kDa