螺旋一环一螺旋蛋白5抗体
规格：1mg/1ml
英文名： BHLHB5
别名： BETA 3; Basic helix loop helix protein 5; Beta3; BHE22_HUMAN; bHLHB5; bHLHe22; CAGL85; Class B basic helix-loop-helix protein 5; Class E basic helix-loop-helix protein 22; Class II bHLH protein BHLHB5
分子量： 37kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human BHLHB5
交叉反应：Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit,
细胞定位：细胞核
螺旋一环一螺旋蛋白5抗体产品介绍：background: Members of the myogenic determination family are basic helix-loop-helix (bHLH) proteins that can be separated into two classes. Class A proteins include the ubiquitously expressed E-box binding factors E12/E47, ITF2 and HEB (BETA 1 or HTF4). Class B proteins such as Myo D, myogenin and Neuro D (BETA 2) are transiently expressed and exhibit a more limited tissue distribution. Class A proteins heterodimerize with class B proteins to activate transcription. Working in opposition to these positively acting factors are a specialized group of proteins that function as dominant negative regulators. For instance, the Id family of transcriptional repressors contains a HLH region required for dimerization but lacks a functional DNA-binding domain. The Id family can therefore form heterodimers with the myogenic family, 螺旋一环一螺旋蛋白5抗体but the resulting complexes are transcriptionally inactive. BETA 3 is a protein that is functionally similar to members of the Id family in that it can inhibit the binding of E47 homodimers as well as E47/Neuro D and E47/Myo D heterodimers to consensus DNA sequences . In contrast to members of the Id family, BETA 3 contains a putative DNA-binding domain. Function: Inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. Despite the presence of an intact basic domain, does not bind to DNA. Subcellular Location: Nucleus. Tissue Specificity: 螺旋一环一螺旋蛋白5抗体Brain-specific, with the highest expression in the cerebellum. Similarity: Contains 1 basic helix-loop-helix (bHLH) domain. Database links: Entrez Gene: 27319 Human Entrez Gene: 59058 Mouse Entrez Gene: 365748 Rat Omim: 613483 Human SwissProt: Q8NFJ8 Human SwissProt: Q8C6A8 Mouse Unigene: 388788 Human Unigene: 591870 Human Unigene: 149938 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
螺旋一环一螺旋蛋白5抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 神经生物学 信号转导 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid