GRC5蛋白抗体
规格：1mg/1ml
英文名： GRC5
别名： CENP-35; GRC5; PHF2; JHDM1E; Jumonji C domain containing histone demethylase 1E; jumonji containing protein; Lysine-specific demethylase PHF2; PHD finger protein 2; Phf2; PHF2_HUMAN.
分子量： 121kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human GRC5/PHF
交叉反应：Human, Mouse, Rat, Chicken, Horse, Sheep,
细胞定位：细胞核
GRC5蛋白抗体产品介绍：background: PHF2 is a 1,101 amino acid protein belonging to the PHD finger protein family. Members of the PHD finger protein family function as transcriptional regulators that affect gene expression by modulating chromatin structure. Expressed in a wide variety of tissues, PHF2 localizes to the nucleus and contains one PHD-type zinc finger and one JMJC domain, suggesting a possible role for PHF2 in transcription regulation and chromatin remodeling. The gene encoding PHF2 lies in the candidate region for hereditary sensory neuropathy type I (HSN1), a disorder characterized by sensory dysfunction. PHF2 exhists as two isoforms produced by alternative splicing, designated isoform GRC5蛋白抗体1 and 2 respectively. Function: Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA. Subunit: Component GRC5蛋白抗体of the PHF2-ARID5B complex, at least composedof PHF2 and ARID5B. Interacts with HNF4A and NR1H4. Subcellular Location: Nucleus, nucleolus. Tissue Specificity: Widely expressed, including in liver (at protein level). Post-translational modifications: Phosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex. Similarity: Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily. Contains 1 JmjC domain. Contains 1 PHD-type zinc finger. Database links: Entrez Gene: 5253 Human Entrez Gene: 18676 Mouse Entrez Gene: 306814 Rat Omim: 604351 Human SwissProt: O75151 Human SwissProt: Q9WTU0 Mouse Unigene: 212554 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
GRC5蛋白抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 神经生物学 信号转导 转录调节因子 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid