神经细胞鸟苷酸置换因子NGEF抗体
规格：1mg/1ml
英文名： Ephexin-1
别名： ARHGEF27; Eph-interacting exchange protein; EPHEXIN; Ephexin 1; Neuronal guanine nucleotide exchange factor; Ngef; NGEF_HUMAN.
分子量： 82kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human EPHEXIN
交叉反应：Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse,
细胞定位：细胞浆 细胞膜
神经细胞鸟苷酸置换因子NGEF抗体产品介绍：background: EPHEXIN is a 710 amino acid protein that localizes to both the membrane and the cytoplasm and contains one SH3 domain, one PH domain and one DH domain. Expressed at high levels in brain and present at lower levels in lung tissue, EPHEXIN interacts with EphA4 and functions as a guanine nucleotide exchange factor (GEF) that is capable of activating Rho A, Rac 1 and Cdc42 and is thought to play a role in axon guidance and growth cone collapse. EPHEXIN is subject to Src-dependent phosphorylation, an event that increases the GEF activity of EPHEXIN toward Rho A. Human EPHEXIN, which exists as multiple alternatively spliced isoforms, shares a high degree of sequence homology with its mouse counterpart, suggesting a conserved role between species. Function: Acts as a guanine nucleotide神经细胞鸟苷酸置换因子NGEF抗体 exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1-and CDC42-stimulated growth cone extension. Subunit: Interacts with CDK5R1 and EPHA4; activated by EPHA4 through the CDK5 kinase Subcellular Location: Cytoplasm. Membrane. Cell projection, growth cone. 神经细胞鸟苷酸置换因子NGEF抗体Associated with membranes. Localizes to axonal growth cones. Tissue Specificity: Highly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung. Post-translational modifications: Src-dependent phosphorylation at Tyr-179 upon EPHA4 activation increases the guanine exchange factor activity toward RHOA. Similarity: Contains 1 DH (DBL-homology) domain. Contains 1 PH domain. Contains 1 SH3 domain. Database links: Entrez Gene: 25791 Human Entrez Gene: 53972 Mouse Entrez Gene: 246217 Rat Entrez Gene: 424744 Chicken Omim: 605991 Human SwissProt: Q8N5V2 Human SwissProt: Q8CHT1 Mouse SwissProt: Q5BKC9 Rat Unigene: 97316 Human Unigene: 435439 Mouse Unigene: 20670 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
神经细胞鸟苷酸置换因子NGEF抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 神经生物学 信号转导 G蛋白偶联受体 G蛋白信号
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid