微小染色体维持缺陷蛋白4抗体
规格：1mg/1ml
英文名： MCM4
别名： CDC21; CDC21 homolog; CDC54; DNA replication licensing factor MCM 4; DNA replication licensing factor MCM4; hCdc 21; hCdc21; Homolog of S. pombe cell devision cycle 21; KIAA4003; mcdc21; MCM 4; MCM 4
分子量： 96kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human MCM4
交叉反应：Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,
细胞定位：细胞核
微小染色体维持缺陷蛋白4抗体产品介绍：background: The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, 微小染色体维持缺陷蛋白4抗体results in the inactivation of the MCM complex (composed of at least MCM proteins 2-6) during apoptosis. Function: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker 微小染色体维持缺陷蛋白4抗体A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Subunit: Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (By simililarity). Interacts with MCMBP. Subcellular Location: Nucleus. Similarity: Belongs to the MCM family. Contains 1 MCM domain. Database links: Entrez Gene: 4173 Human Entrez Gene: 17217 Mouse Omim: 602638 Human SwissProt: P33991 Human SwissProt: P49717 Mouse Unigene: 460184 Human Unigene: 1500 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
微小染色体维持缺陷蛋白4抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 细胞凋亡 细胞周期蛋白 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid